Buffer | Filtration (F) / Autoclaving (A) | Conditioning |
TBS 10X pH=7.5248mM Tris - 1.37M NaCl - 27mM KCl | A | 1 L |
TAE 50X 2M Tris - 50mM EDTA - 5.7% acetic acid | 1 L | |
TBE 10X 890mM Tris - 890mM boric acid - 20mM EDTA | 1 L | |
Tris-Glycine running buffer 5X 250mM Tris - 2.5M Glycine - 0.5% SDS | 2 L | |
Tris Resolving buffer pH=8.8 1.5M Tris pH=8.8 | F | 0.5 L |
Tris Stacking buffer pH=6.8 500mM Tris pH=6.8 | F | 0.5 L |
Western blot Transfer buffer 10X 250mM Tris - 1.9M Glycine | 2 L | |
TE 10X pH=7.5100mM Tris-HCl - 10mM EDTA | F | 0.1 L |
NaCl 5M | F | 0.5 L |
KCl 3M | F | 0.25 L |
Tris 1M various pH (buffered with HCl) | F | 0.5 L |
Hepes 1M various pH (buffered with KOH) | F | 0.25 L |
KH2PO4 / K2HPO4 0.2M | F | 0.25 L |
Na2HPO4 / NaH2PO4 0.2M | F | 0.25 L |
EDTA 0.5M | F | 0.25 L |
MgCl2 1M | F | 0.1 L |
CaCl2 1M | F | 0.1 L |
MgSO4 1M | F | 0.1 L |
Glycérol | 0,1 L |
Medium | Filtration (F) / Autoclaving (A) | Conditioning |
Medium | Antibiotic | Color |
LB Lennox Agar 5g NaCl – 5g yeast extract – 10g bactotryptone - 12.5g Bacto agar for 1 L | Ampicillin (100µg/ml) | Black | Ask the service to see the performed tests |
LB Lennox Agar 5g NaCl – 5g yeast extract – 10g bactotryptone - 12.5g Bacto agar for 1 L | Kanamycin (50µg/ml) | Red | Ask the service to see the performed tests |
LB Lennox Agar 5g NaCl – 5g yeast extract – 10g bactotryptone - 12.5g Bacto agar for 1 L | Chloramphenicol (34µg/ml) | Blue | Ask the service to see the performed tests |
LB Lennox Agar 5g NaCl – 5g yeast extract – 10g bactotryptone - 12.5g Bacto agar for 1 L | Spectinomycin (50µg/ml) | Green | Ask the service to see the performed tests |
LB Lennox Agar 5g NaCl – 5g yeast extract – 10g bactotryptone - 12.5g Bacto agar for 1 L | Gentamycin (30µg/ml) | Purple | Ask the service to see the performed tests |